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methyltransferase  (New England Biolabs)


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    Structured Review

    New England Biolabs methyltransferase
    Methyltransferase, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 227 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/dam+methyltransferase/dam+Methyltransferase/pmc13112749-355-33-34
    Average 95 stars, based on 227 article reviews
    methyltransferase - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The yeast genome is globally accessible in living cells.
    Article Snippet: .. Dam methyltransferase (New England Biolabs (NEB) M0222L, 8 units per μl; 1.6 μg Dam per ml) was added (0, 50, 100, 200 or 400 units, final concentration in nM 0, 0.77, 1.5, 2.9, 5.6) to the nuclei, mixed gently and incubated for 20 min at 25 °C. ..

    Incubation:

    Article Title: The yeast genome is globally accessible in living cells.
    Article Snippet: .. Dam methyltransferase (New England Biolabs (NEB) M0222L, 8 units per μl; 1.6 μg Dam per ml) was added (0, 50, 100, 200 or 400 units, final concentration in nM 0, 0.77, 1.5, 2.9, 5.6) to the nuclei, mixed gently and incubated for 20 min at 25 °C. ..

    Article Title: Nucleosome dynamics render heterochromatin accessible in living human cells
    Article Snippet: .. Dam methyltransferase (NEB M0222B-HC2 at 40 U/μl; 8 μg Dam/ml) was added to the aliquots of nuclei: 0, 25, 50, 100, and 200 units (0, 0.8, 1.6, 3.1, 6.3 nM, respectively), gently mixed, and incubated for 30 min at 37°C. .. Genomic DNA was extracted using the PureLink Genomic DNA Kit (Invitrogen 2666617).

    Article Title: Nucleosome dynamics render heterochromatin accessible in living human cells
    Article Snippet: .. Dam methyltransferase (NEB M0222B-HC2 at 40 U/μl; 8 μg Dam/ml) was added to the aliquots of nuclei: 0, 25, 50, 100, and 200 units (0, 0.8, 1.6, 3.1, 6.3 nM, respectively), gently mixed, and incubated for 30 min at 37 °C. .. Genomic DNA was extracted using the PureLink Genomic DNA Kit (Invitrogen 2666617).

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table ) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table ), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s‐adenosyl‐methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis.
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table S1) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table S1), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s- adenosyl- methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Methylation:

    Article Title: Centromeres and pericentromeric heterochromatin are accessible in living Schizosaccharomyces pombe cells
    Article Snippet: .. Methylation reactions were performed by adding 0, 25, 50, 100 or 200 U Dam methyltransferase (NEB M0222L at 8 U/μl; 1.6 μg/ml) to 100 μl aliquots of nuclei (final Dam concentrations: 0, 1.5, 2.9, 5.6 and 10.0 nM) and incubating at 25°C for 30 min. ..

    Polymerase Chain Reaction:

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table ) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table ), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s‐adenosyl‐methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis.
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table S1) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table S1), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s- adenosyl- methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Purification:

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table ) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table ), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s‐adenosyl‐methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis.
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table S1) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table S1), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s- adenosyl- methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Recombinant:

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table ) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table ), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s‐adenosyl‐methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.

    Article Title: Highly sensitive detection of DNA methyltransferase1 triggered by methylation protection
    Article Snippet: Type 2 diabetes is on the rise year by year, and 80 % of patients with type 2 diabetes die from cardiovascular complications.. Strict control of blood glucose can significantly reduce the occurrence and development of diabetic microvascular complications, but can not effectively prevent the occurrence of diabetic macrovascular lesions.. DNA methyltransferase 1 (DNMT1) plays an important role in developing phenotypic switching and secretion of inflammatory factors in vascular smooth muscle cells(VSMC), leading to the deregulation of vascular function.

    Article Title: Targeted Deletion of the Cytopathogenic Toxin A Gene in Sneathia vaginalis.
    Article Snippet: A region of the CptA_KO_erm plasmid was amplified by PCR using primers TestPCRFWD and TestPCRREV (Table S1) and Phire DNA polymerase master mix (Thermo). .. The PCR product, which contained a single, central GATC site (Table S1), was purified using the PCR purification kit (Qiagen), and 1 μg of the purified test substrate was incubated with 3 μg (10 nM) purified recombinant VC03_04590 or 2.5 U of commercially available Dam methyltransferase (New England Biolabs), in the presence of 80 μM s- adenosyl- methionine and 1X methyltransferase buffer (NEB) in 10 μL total reaction volume for 2 h in a 37°C water bath. .. The test substrate was purified using a PCR purification kit (Qiagen), and 1 μg was incubated with 3 μg (10 nM) purified recombinant VC03_04585 or 2 U DpnII, in 1X rCutSmart (NEB) for 2 h at 37°C.



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